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    CRL-9078 PA 317 小鼠成纖維細胞

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    產品名稱: CRL-9078 PA 317 小鼠成纖維細胞
    產品型號: CRL-9078
    產品廠商: 美國標準生物品收藏中心(ATCC)
    產品文檔: 無相關文檔


    簡單介紹

    CRL-9078 PA 317 小鼠成纖維細胞,原代細胞|細胞系|細胞株|菌種;細胞庫管理規范,提供的細胞株背景清楚,提供參考文獻和*優培養條件!


    CRL-9078 PA 317 小鼠成纖維細胞 的詳細介紹

    CRL-9078 PA 317 小鼠成纖維細胞
    ATCC® Number:  CRL-9078™    
    Designations:  PA317 
    Depositors:   Fred Hutchinson Cancer Res. Cntr. 
    Biosafety Level: 1 
    Shipped:  frozen 
    Medium & Serum:  See Propagation 
    Growth Properties: adherent
    Organism: Mus musculus (mouse) 
    Morphology: fibroblast 
    Source: Organ: embryo
    Strain: NIH/Swiss
    Permits/Forms: In addition to the MTA mentioned above, other ATCC and/or regulatory permits may be required for the transfer of this ATCC material. Anyone purchasing ATCC material is ultimately responsible for obtaining the permits. Please click here for information regarding the specific requirements for shipment to your location. 
     
    Applications: transfection host (Roche FuGENE® Transfection Reagents)
    Packaging line for retroviruses.
    Age:  embryo 
    Comments: Derived from TK- NIH/3T3 cells by cotransfection with packaging construct DNA, pPAM3, carried in pBR322 and herpes simplex virus thymidine kinase (TK) gene carried in pBR322.
    The PA317 cell line was derived from TK- NIH/3T3 cells by cotransfection with packaging construct DNA (pPAM3) carried in pBR322 and the herpes simplex virus thymidine kinase (TK) gene carried in pBR322.
    Introduction of retroviral vectors into these cells, by infection or by transfection, results in production of retrovirus virions with an amphotropic host range that are capable of infecting cells of many mammalian species.
    Virions produced by this line have been used successfully to transfer genes into humans.
    The percentage of PA317 cells that are capable of packaging retroviral vectors decreases slowly with continued passaging of the cell line, presumably due to the loss of the transfected DNA used to create the line.
     

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