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    CCL-9.1 NCTC 1469 小鼠正常肝細胞

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    產品名稱: CCL-9.1 NCTC 1469 小鼠正常肝細胞
    產品型號: CCL-9.1
    產品廠商: 美國標準生物品收藏中心(ATCC)
    產品文檔: 無相關文檔


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    CCL-9.1 NCTC 1469 小鼠正常肝細胞,原代細胞|細胞系|細胞株|菌種;細胞庫管理規范,提供的細胞株背景清楚,提供參考文獻和*優培養條件!


    CCL-9.1 NCTC 1469 小鼠正常肝細胞 的詳細介紹
    CCL-9.1 NCTC 1469 小鼠正常肝細胞
    ATCC® Number: CCL-9.1™    Price: $329.00
    Designations: NCTC clone 1469 [derivative of NCTC 721]
    Depositors:  VJ Evans
    Biosafety Level: 1
    Shipped: frozen
    Medium & Serum: See Propagation
    Growth Properties: adherent
    Organism: Mus musculus (mouse)
    Morphology: epithelial

    Source: Organ: liver
    Strain: C3H/An
    Disease: normal
    Permits/Forms: In addition to the MTA mentioned above, other ATCC and/or regulatory permits may be required for the transfer of this ATCC material. Anyone purchasing ATCC material is ultimately responsible for obtaining the permits. Please click here for information regarding the specific requirements for shipment to your location.
     
    Isolation: Isolation date: April, 1952
    Virus Susceptibility: Vesicular stomatitis virus
    Human poliovirus 1
    Antigen Expression: H-2k
    Cytogenetic Analysis: modal number = 41; range = 38 to 86.
    Two marker chromosomes present: One metacentric with one pair of arms having achromatic gaps near centromere; and an acrocentric with satellites.
    Age: newborn
    Gender: male
    Comments: Tested and found negative for ectromelia virus (mousepox).
    Propagation: ATCC complete growth medium: The base medium for this cell line is ATCC-formulated Dulbecco's Modified Eagle's Medium, Catalog No. 30-2002. To make the complete growth medium, add the following components to the base medium: horse serum to a final concentration of 10%.
    Atmosphere: air, 95%; carbon dioxide (CO2), 5%
    Temperature: 37.0°C
    Subculturing: Protocol: Subcultures are prepared by scraping. Remove old medium, add fresh medium and dislodge the cells from the floor of the flask. Aspirate cells and dispense the suspension into new flasks.
    A standard trypsinizaton may be used if desired.
    The culture medium in heavy cultures may be turbid in appearance and and give the gross impression of bacterial contamination.
    It is characteristic of this line to shed viable cells into the medium, thus rendering the medium turbid. The shed cells are viable and may be used to initiate new cultures.
    Subcultivation Ratio: A subcultivation ratio of 1:2 to 1:4 is recommended
    Medium Renewal: 3 times per week
    Preservation: Freeze medium: Complete growth medium supplemented with 5% (v/v) DMSO
    Storage temperature: liquid nitrogen vapor phase
    Related Products: Recommended medium (without the additional supplements or serum described under ATCC Medium):ATCC 30-2002
    recommended serum:ATCC 30-2040
    References: 22167: Hobbs GL, et al. Establishment of a clone of mouse liver cells from a single isolated cell. J. Natl. Cancer Inst. 18: 701-707, 1957. PubMed: 13502690
    26070: Evans VJ, et al. The growth in vitro of massive cultures of liver cells. J. Natl. Cancer Inst. 12: 1245-1265, 1952. PubMed: 14939026
    26074: . . J. Natl. Cancer Inst. 23: 823, 1959.
    26076: . . J. Natl. Cancer Inst. 27: 29, 1961.
    26078: Evans VJ, et al. Studies on culture lines derived from mouse liver parenchymatous cells grown in long-term tissue culture. Cancer Res. 12: 261-266, 1958. PubMed: 13523589
    26079: . . Fed. Proc. 17: 967, 1958.
    26081: Westfall BB. Characterization of cells in tissue culture. Natl. Cancer Inst. Monogr. 7: 147-158, 1962. PubMed: 14006338
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