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    OKT 3 雜交瘤(抗CD3)

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    產品名稱: OKT 3 雜交瘤(抗CD3)
    產品型號: CRL-8001
    產品廠商: 美國標準生物品收藏中心(ATCC)
    產品文檔: 無相關文檔


    簡單介紹

    CRL-8001 OKT 3 雜交瘤(抗CD3) ATCC 細胞|細胞系|細胞株|腫瘤細胞|細胞;細胞庫管理規范,提供的細胞株背景清楚,提供參考文獻和*優培養條件!


    OKT 3 雜交瘤(抗CD3) 的詳細介紹
    CRL-8001 OKT 3 雜交瘤(抗CD3)
    ATCC® Number: CRL-8001? Price:
    Designations: OKT 3
    Depositors: Ortho Pharmaceutical Corporation
    Isotype: IgG2a
    Biosafety Level: 1
    Shipped: frozen
    Medium & Serum: See Propagation
    Growth Properties: suspension
    Organism: Mus musculus (B cell); Mus musculus (myeloma)
    Morphology: lymphoblast

    Source: Cell Type: hybridoma: B lymphocyte;
    Cellular Products: immunoglobulin; monoclonal antibody; against human T cell (human T lymphocyte); against CD3
    Permits/Forms: In addition to the MTA mentioned above, other ATCC and/or regulatory permits may be required for the transfer of this ATCC material. Anyone purchasing ATCC material is ultimately responsible for obtaining the permits. Please click here for information regarding the specific requirements for shipment to your location.
     
    Comments: Mice were immunized with human peripheral blood lymphocytes.
    Spleen cells were fused with P3x63Ag8.U1 myeloma cells.
    Propagation: ATCC complete growth medium: The base medium for this cell line is ATCC-formulated Iscove's Modified Dulbecco's Medium, Catalog No. 30-2005. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 20%.
    Temperature: 37.0°C
    Subculturing: Medium Renewal: Every 2 to 3 days
    Cultures can be maintained by addition or replacement of fresh medium. Start cultures at 2 X 10 exp5 cells/ml and maintain between 1 X 10 exp5 and 1 X 10 exp6 cells/ml.
    Related Products: Recommended medium (without the additional supplements or serum described under ATCC Medium):ATCC 30-2005
    recommended serum:ATCC 30-2020
    References: 980: Hoffman RA, et al. Simple and rapid measurement of human T lymphocytes and their subclasses in peripheral blood. Proc. Natl. Acad. Sci. USA 77: 4914-4917, 1980. PubMed: 6968909
    3499: Kung PC, Goldstein G. Complement-fixing monoclonal antibody to human T cells, and methods of preparing same. US Patent 4,361,549 dated Nov 30 1982
    28435: Tanaka Y, et al. Productive and lytic infection of human CD4+ type 1 helper T cells with macrophage-tropic human immunodeficiency virus type 1. J. Virol. 71: 465-470, 1997. PubMed: 8985372
    32767: Solomon KR, et al. The association between glycoslphosphatidylinositol-anchored proteins and hetrotrimeric G protein alpha subunits in lymphocytes. Proc. Natl. Acad. Sci. USA 93: 6053-6058, 1996. PubMed: 8650218
    33089: Hutchcroft JE, et al. Differential phosphorylation of the T lymphocyte costimulatory receptor CD28. J. Biol. Chem. 271: 13362-13370, 1996. PubMed: 8662792
    33136: Hartley D, Corvera S. Formation of c-Cb1-phosphatidylinositol 3-kinase complexes on lymphocyte membranes by a p56lck-independent mechanism. J. Biol. Chem. 271: 21939-21943, 1996. PubMed: 8702998
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